Journal:

Article Title: Calprotectin Expression by Gingival Epithelial Cells

doi: 10.1128/IAI.69.5.3248-3254.2001

Figure Lengend Snippet: Treatment of immortalized keratinocytes with IL-1β (0.1, 1, and 10 ng/ml) for 2 or 4 h. Immortalized keratinocytes were incubated with various concentrations of IL-1β (0.1, 1, and 10 ng/ml) for 2 h (black bars) or 4 h (crosshatched bars). Expression of mRNA for IL-8-, MRP8-, and MRP14-specific mRNA was determined by RNase protection assay. The signal intensity for the protected fragments was normalized to the abundance of the internal control GAPDH. The data shown are the mean ± the standard deviation (n = 3).

Article Snippet: Immortalized keratinocytes were stimulated by incubation with lipopolysaccharide from E. coli O55:B5 (Sigma, St. Louis, Mo.), phorbol 12-myristate 13-acetate (PMA; Sigma) or recombinant human IL-1β (R&D Systems, Inc., St. Paul, Minn.).

Techniques: Incubation, Expressing, Rnase Protection Assay, Standard Deviation

Journal: Frontiers in Immunology

Article Title: Blocking LAIR1 signaling in immune cells inhibits tumor development

doi: 10.3389/fimmu.2022.996026

Figure Lengend Snippet: Inhibition of LAIR1 enhances dendritic cell differentiation and dendritic cell- induced T cell proliferation in MLR assay. (A) CD14 + monocytes isolated from fresh human PBMCs were cultured with GM-CSF and IL-4 for 2 days. The expression of CD11c on CD14 negative population and CD86 were detected. (B, C) Matured DCs were mixed with allogenic T cells in the MLR assay. Surface CD25 (B) and CFSE based cell proliferation were determined by flow cytometry at 72 hours (C). As Statistical Analysis in Methods stated: * indicates p < 0.05; ** indicates p < 0.01, *** indicates p < 0.001.

Article Snippet: 1x10 5 CD14 + cells were cultured in RPMI 1640 medium (R8758-500ml, Sigma) supplemented with 10% fetal bovine serum (F0926, Sigma), 2 mM L-alanine-L-glutamine (SH3003402, Fisher), 50 ng/ml GM-CSF (300-03, PeproTech) and 25 ng/ml human IL-4 (11846-HNAE-1, Sino Biological) per well of a 96-well plate for 6 days; fresh medium was replaced every 3 days.

Techniques: Inhibition, Cell Differentiation, Mlr Assay, Isolation, Cell Culture, Expressing, Flow Cytometry

Journal:

Article Title: Cytokine response to vitamin E supplementation is dependent on pre-supplementation cytokine levels

doi:

Figure Lengend Snippet: Description of SNPs, primers and probes

Article Snippet: IL-1β was detected using mouse anti-human IL-1β MAb and biotinylated anti-human IL-1β Ab (R&D Systems, Minneapolis, MN).

Techniques:

Journal:

Article Title: Cytokine response to vitamin E supplementation is dependent on pre-supplementation cytokine levels

doi:

Figure Lengend Snippet: Genotype frequencies

Article Snippet: IL-1β was detected using mouse anti-human IL-1β MAb and biotinylated anti-human IL-1β Ab (R&D Systems, Minneapolis, MN).

Techniques:

Journal:

Article Title: Cytokine response to vitamin E supplementation is dependent on pre-supplementation cytokine levels

doi:

Figure Lengend Snippet: The effect of vitamin E supplementation on cytokine production depends on baseline cytokine production. Cytokine production was measured from whole blood at the beginning and end of a one year vitamin E supplementation in the elderly. TNF-α, IL-1β, and IL-6 was measured from whole blood elicited for 24 hours with lipopolysacchride (LPS; 1.0 µg/mL). IFN-γ was measured from whole blood elicited for 48 hours with phytohemagluttinin (PHA; 20 µg/mL) or concalavinA (ConA; 40 µg/mL). Cytokines production was corrected for the number of monocytes and lymphocytes in the blood (pg/ 106 lymphocyte and monocyte). P values (P) for the interaction are unadjusted.

Article Snippet: IL-1β was detected using mouse anti-human IL-1β MAb and biotinylated anti-human IL-1β Ab (R&D Systems, Minneapolis, MN).

Techniques: